Device

Part:BBa_J64032:Design

Designed by: Dan Widmaier   Group: Voigt Lab   (2008-01-16)


pCASP SPI-1 Secretion Circuit


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1767
    Illegal PstI site found at 533
    Illegal PstI site found at 1158
    Illegal PstI site found at 1388
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 533
    Illegal PstI site found at 1158
    Illegal PstI site found at 1388
    Illegal NotI site found at 1684
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1478
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1767
    Illegal PstI site found at 533
    Illegal PstI site found at 1158
    Illegal PstI site found at 1388
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1767
    Illegal PstI site found at 533
    Illegal PstI site found at 1158
    Illegal PstI site found at 1388
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1632


Design Notes

This device was not designed with biobrick standard assembly in mind and has several of the scar sequence sites throughout the circuit.


Source

This is a composite part consisting of both natural and synthetic DNA components

References